The LeviCell system consistently rescued viable cells from low-quality suspensions containing dead cells and debris providing usable samples in instances where the Miltenyi Dead Cell Removal Kit failed. The system delivered high performance across a range of cell inputs of 18,500 to 7 million cryopreserved cells with starting viability from 16% to 82%. The routine use of the LeviCell system ensures successful analysis of viable cells across a wide range of starting samples of varying quality quantity and sample types, such as PBMC and DTC-derived single-cell suspensions.

Single-cell RNA sequencing (scRNA-seq) is a powerful tool that can help researchers uncover rare and complex cell populations, evaluate the role of certain genes and/or screen drugs for their effect on different cell types in the context of numerous disorders. In this research snapshot, we demonstrate how the quality of your downstream scRNA-seq data and analysis can be affected by poor-quality samples and how a simple enrichment step can change everything.