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Flow cytometry analysis can be confounded by low cell numbers, low cell viability and contaminants such as cellular debris. Sample optimization with levitation technology can address these challenges simultaneously.
Access to all cells is important when studying the tumor microenvironment. However, characterization of genetic modulations in tumor cells often require removal of immune cells. Levitation Technology enables depletion of immune cells and simultaneous enrichment for viable tumor cells in one run.
These protocols provide guidance on how to work with both perfused and non-perfused adult mouse brain, and illustrates how one can enrich for either microglia only or all neural cell types using the LeviCell System.
Isolation and interrogation of single cells from fresh brain tissue is crucial for scientists to understand their role in homeostasis, disease conditions and drug treatments. This application note illustrates how the LeviPrep-LeviCell workflow delivers enriched homeostatic microglia with 2-6 fold decrease in microglia activation markers than alternative isolation methods.
The quality of downstream scRNA-seq data and analysis can be degraded by poor quality samples. Learn how a simple enrichment step can improve your QC metrics, allow you to retain 2.5 fold more cells, and decrease ambient RNA contamination by 1.7 fold.
LevitasBio has built a solution for cellular enrichment and characterization around proprietary magnetic levitation technology. At its core, this technology is driven not only by the LeviCell instrument itself, but through its single-use consumable, the LeviCell cartridge. The new S2.3 cartridge design offers better visualization of levitating cells which enables powerful sample characterization through advanced software analytics while providing equivalent performance in sample enrichment.